5'-Nucleotidase and Gamma-Glutamyl Transpeptidase Activities in the Cholestatic Rat Liver Plasma Membranes; Mitochondria and Microsomes

Keimyung Medical Journal 1987;6(1):67-76.

흰쥐 담즙울체간의 Plasma Membrance; Mitochondria 및 Microsome의 5'-Necleotidase와 Gamma-Glutamyl Transpeptidase의 활성치

곽춘식; 김여희; 문교철

Abstract

Changes in the activities of the following have been studied over a period of forty two days following the ligation of common bile duct in rats: plasma membrane; microsomal; mitochondrial; nuclear and cytosolic 5'-nucleotidase and gamma-glutarnvl transpeptidase of cholestatic liver. The activities of alkaline phosphatase in the subcellular fractions of cholestatic liver were also measured. The activities of microsomal and cytosolic 5'-nucleotidase in cholestatic liver markedly elevated between the second and the forty-second days of the operation. However; the plasma membrane; mitochondrial and nuclear 5'-nucleotidase activities in the cholestatic liver showed a substantial decrease from three days to forty two days after the ligation of common bile duct. The activities of hepatic microsomal; cytosolic; plasma membrane; mitochondrial and nuclear gamma-glutamyl transpeptidase drastically increased throughout the experiments. The activities of microsomal and cytosolic alkaline phosphatase in the cholestatic liver strikingly increased during the forty two days following the operation; but the plasma-membrane bound alkaline phosphatase in the cholestatic liver had a significant diminution after the ligation of ?common bile duct.