Immunohistologfical Demonstration of Fibronectin in Human Granulation Tissues

Keimyung Medical Journal 1987;6(1):60-66.

육아조직의 fibronectin의 면역 조직학적 검색

이상숙; 정재홍; 박준식

Abstract

This study describes the distribution of fibronectin and its association with reticulin fibers (type U collagen) in incisional wounds and previous biopsy sites of randomly sampled patients. The specimens were examined using immunofluorescence and immunoperoxidase to localize affinity-purified antibodies to fibronectin. Tissue samples were also stained with hematoxylin and eosin; and reticulum stain. Fibronectin was observed in the matrix surrounding individual fibroblasts and codistributed with reticulin fibers in early granulation tissue. Dense fibrillar networks of fibronectin and fibroblasts; giving the granulation tissue a highly stuctured and organized appearance. When the collagen had matured into bundles; fibronection dimimished or disappeared. These findings suggest that fibronectin may be important by functioning as a primary matrix for organization of the collagenous connective tissue during the tissue repair process.