| Co-expression of Cholecystokinin and Neuropeptide Y by Glutamatergic Stimulation in PC12 Cells |
| Co-expression of Cholecystokinin and Neuropeptide Y by Glutamatergic Stimulation in PC12 Cells |
|
권지윤; 김수경 |
|
|
|
|
| Abstract |
|
Co-expression of cholecystokinin (CCK) and neuropeptide Y (NPY) by glutamatergic stimulation was examined by double stained immnocytochemistry in the rat PC12 cells. Co-expression of CCK and NPY was inducible by glutamate and N-methyl-D-aspartate (NMDA); but not by kainic acid. Pretreatment of the cells with non-competitive NMDA antagonist; MK-801 (10 ?M); and competitive NMDA antagonist; AP5 (10 ?M); slightly suppressed the elevated levels of glutamate-induced CCK and NPY immunoreactivity; but markedly inhibited those of NMDA-induced CCK and NPY immunoreactivity. These results suggest that CCK and NPY expressions are mainly regulated through the NMDA-induced signaling pathways; and both molecules could be co-expressed via NMDA receptors upon glutamatergic neuroexcitation at the synaptic junction. |
| Key Words:
Cholecystokinin, Glutamatergic stimulation, Neuropeptide Y, PC12 cell |
PDF Links
Full text via DOI
Download Citation
