Effect of 
Hematopoietic Growth Factors on the Generation of Megakaryocyte Colony Forming Units (CFU-MK)

Keimyung Medical Journal 2003;22(2):250-258.

Effect of Hematopoietic Growth Factors on the Generation of Megakaryocyte Colony Forming Units (CFU-MK)

골수 단핵세포의 거핵구 집락 배양에 대한 조혈성장인자의 효과

장용석; 권기영

Abstract

In vitro colony assay procedures for expansion of human erythroid and granulocyte-macrophage progenitors have been used for many years. However; the development of reproducible assay method for human megakaryocyte (MK) progenitors considerably lagged behind. Cytokines such as stem cell factor (SCF); thrombopoietin (TPO); IL?6 and IL-11 have been demonstrated to have effects on the production of colony forming units (CFU)-MK. This study was undertaken to investigate the ex vivo expansion of megakaryocyte progenitors; derived from mononuclear cells of normal tone marrow. Bone marrow mononuclear cells were incubated in serum-free medium (MegaCult™; Stem Cell Technologies; Vancouver; Canada) and supplemented with SCF; granulocyte-colonystimulating factor (G-CSF); TPO; IL-11; combinations of SCF + G- CSF + TPO and SCF + G-CSF + TPO + IL-11. The effect of hematopoietic growth factors on ex vivo expansion of megakaryocyte progenitor cells was evaluated. These cytokines were able to generate of CFU-MK in the media and to increase megakaryocyte colony formation: Numbers of pure MK colonies increased in the media supplemented with SCF; IL-11 and combination of cytokines. Mixed MK colonies also increased in the media supplemented with SCF; G-CSF and combination groups.

Key Words: Megakaryocyte, Hematopoietic grwoth factor, Thrombopoietin, IL ? 6, IL ? 11, Stem cell factor