| Lectin Histochemistry
of Degenerating and Regenerating Taste Buds in Rat
Circumvallate Papilla |
| 흰쥐 성곽유두 맛봉오리의 퇴화와 재생과정에 따른 렉틴조직화학 |
|
문용석; 김욱; 정효순; 최인장 |
|
|
|
|
| Abstract |
|
The present study was carried
out to investigate the structure and glycoconjugate properties of degenerating and regenerating taste buds in the circumvallate papilla. The bilateral glossopharyngeal nerves of Sprague-Dawley male rats (300-350 g) were crushed by tweezers for 15 seconds. The animals were sacrificed 1; 4; 7; 10 days; 2; 3; 4 and 6 weeks later. The circum vallate papilla of the tongue was dissected; the tissues were then fixed with 4% paraformaldehyde solution; and tissue samples after hematoxylin-eosin staining were examined for morphological changes. They were also reacted with 8 biotinylated lectins: Con A; PSA; UEA I; ECL; DBA; HP A; SJA and sWGA. In the control; the taste buds in the circumvallate papilla were oval shape; and the two types of cells; round pale-staining nuclei and more slender darker-staining nuclei; were present in the taste buds. The taste buds and taste cells were extremely atrophied in the 4 days group; and a large number of the taste cells with round nuclei appeared in the 7 days group. The taste buds and taste cells regenerated in the 2 weeks group and they had the structure similar to the control in the 4 weeks group. In the control; strong positive reaction of taste pore and taste cells in the taste buds to UEA I; SBA; HPA and SJA may suggest that the taste cells secrete glycoconjugates which contained <α-L-fucose and N-acetyl-D-galactosamine. The DBA; HPA and SJA positive reactive taste cells decreased in the 7 days group and increased in the 10 days and 4 weeks groups. |
| Key Words:
Circum vallate papilla,
Degeneration, Histochemistry, Lectin, Regeneration, Taste bud |
|
PDF Links
Full text via DOI
Download Citation
