| Effect of IL-4
Expressed by Recombinant Adenovirus
on Collagen Induced Arthritis |
| 콜라겐 유발성 관절염에 대한 IL-4발현 재조합 아레노바이러스의 효과 |
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박종구 |
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| Abstract |
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Interleukin-4 (IL-4) is one of
anti-inflammatory cytokines which has
the potential to counteract pro-inflammatory cytokines often detected in
rheumatoid arthritis. This study examined the effect of adenovirus-mediated gene
transfer of IL-4 on collagen-induced arthritis (CIA); an animal model of
rheumatoid arthritis. The IL-4 cDNA was cloned from a T lymphoma cell line after
induction with PMA and was further cloned into an adenovirus shuttle vector. The
recombinant adenovirus encoding rat IL-4; AdrIL-4; was constructed by
homologous recombination between the cloned shuttle vector and a rescue vector;
pJM17; and identified by polymerase chain reaction. CIA was induced in Dark
agouti (DA) rats by immunizing type II collagen. On 12th day after immunization;
DA rats were administrated with intra-articular injection of 4×108 plaque-forming
units of AdrIL-4. The exogenous IL-4 expression was observed to inhibit CIA
progression; compared to those of control groups; in which PBS or recombinant
adenovirus without a transgene; Adnull; was administered. Whereas any
significant difference was not observed on the production of anti-collagen
antibodies among different groups of rats; IL-4 gene transfer but not with control
treatments was shown to suppress CIA in a moderate manner. |
| Key Words:
Collagen induced arthritis,
Interleukin-4, Recombinant adenovirus |
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