| Induction of
Antiviral Antibody using HBV Gene and HBV mRNA Pulsed
Antigen Presenting Cell |
| B형 간염바이러스 유전자 및 유전자 이입 항원제공세포를 이용한 항바이러스 항체생성 유도 |
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박종욱; 권택규 |
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| Abstract |
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In order to develop effective
vaccine to recover incomplete immunity
in patients with chronic hepatitis B virus infection; we produced various types of
vaccine for Hepatitis B virus core (HBc) antigen and compared their effects on
anti-HBc antibody response. Intramuscular injection of HBc DNA vaccine (pc
-HBc) and intraperitoneal injection of HBc-transfected Renca and EL-4 effectively
induced anti-HBc antibody response in mouse. Lipopolysaccharide (LPS) induced
gene expressions of IP-10; GM-CSF; and IL-12 in dendritic cell (DC). However;
LPS-stimulated DC or peritoneal macrophage; that were trasnsfected with HBc
mRNA; on HBc mRNA vaccine itself induced lower anti-HBc antibody response in
mouse compared to pc-HBc vaccine. These results suggest HBc DNA vaccine is
more simple and effective for the induction of anti-HBc antibody response than
HBc mRNA or mRNA-transfected cell vaccine. |
| Key Words:
Core antigen,
Hepatitis B virus, Vaccine |
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