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Keimyung Medical Journal 1996;15(1):96-104.
Immunogold Labelling in the Transmission and Scanning Electron Microscopy
투과 및 주사전자현미경 영역에서 면역금표지법
권건영
Abstract
Immunocytochemistry at the electron microscopic level has allowed the distribution of antigens in the cellular system to be examined. In transmission immunoelectron microscopy; two different approaches have been used; i. e.; preembedding or post-embedding methods; depending on whether labeling is carried out before or after the embedding process; respectively. The good preservation and expression of the antigenic molecules in the tissues are most important in the ultrastructural immunocytochemistry. A low temperature resin; Lowicryl K4M for postembedding method is frequently used in many immunoelectron microscopic laboratories. Periodate-lysine-pa raformaldehyde(PLP) fixed tissues are dehydrated and embedded at -20°C in a hydrophilic resin; Lowicryl K4M. The resin is easy to section and a good embedding material for preservation of antigenicity in the tissues. Immunogold labelling technique marks localization of antigenic molecules in the subcellular level. In the scanning immunoelectron microscopy; a silver-enhancing technique can well express the antigenic im-munogold particles using smaller gold probes((l5nm) in the standard scanning electron microscopy
Key Words: Post-embedding method, Silver-enhancing, Transmission immunoelectron microscopy, Scanning immunoelectron microscopy
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