Activities of 5'-Nucleotidase and Gamma-Glutamyl Transpeptidase of Cholestatic Liver in Rats

Keimyung Medical Journal 1985;4(1):1-27.

흰쥐 담즙울체 간장의 5'-Nucleotidase와 Gamma-Glutamyl Transpeptidase의 활성치

곽춘식; 장억규

Abstract

Changes in the activities of 5'~nucleotidase, gamma-gultamyl transpetidase, and alkaline phosphatase in serum were studied with rats which had bile duct ligated totally and partially over a period of 6 days after operation. The above enzymes were also observed in the activities of both membrane bound and soluble of liver. Activities of alanine aminotransferase, asparate aminotransferase, and lactate dehydrogenase in both serum and liver, and incorporation rates of amino acid mixture-(U)-uC into both serum protein and liver protein were compared with the changes observed above. And then the effects of actinomycin D adminstered to totally ligated rats were also observed. The levels of total bilirubin of serum increased sharply after the ligation of the common bile duct. 5'-Nucletidase, gamma-glutamyl transpeptidase and alkaline phosphatase showed increased activities both in membrane bound and in soluble of liver, however, the increases of『-nucleotidase and gamma-glutamyl transpeptidase were not detectable until second day, whereas the significant increase of alkaline phosphatase was detectable within 12 hours after common bile duct ligation. In the serum, however, the activities of these enzymes were significantly elevated in 12 hours after operation. Maximum levels were appeared on first day for 5'-nucleotidase and on third day for alkaline phophatase and gamma-glutamyl transpeptidase. 5'-Nucleotidase activities in the serum of both control rats and totally bile duct ligated ones were found to be approximately increased by 1.7 fold 1 day after the administration of actino-mycin D. The activities of hepatic membrane bound and serum hepatic alkaline phosphatase were significantly diminished 1 day after the administration of actinomycin D. The activities of hepatic membrane bound and soluble of both 5?nucleotidase and gamma-glutamyl transpeptidase, and serum gamma-glutamyl transpeptidase did not change 1 day after the adminstration of actinomycin D. But the act ivies of hepatic membrane bound of both 5'-nucIeotidase and gamma-glutamyl transpeptidase were significantly inhbited 1,5 day after the administration of actinomycin D. Partial ligation of bile duct which is the ligation of bile ducts of both median and left lateral lobes, also induced a significant increase of 5'-nucleotidase, gamma-glutamyl transpeptidase, and alkaline phosphatase in serum but serum bilirubin level remained within the normal range. Liver membrane bound of nucleotidase and gamma-glutamyl transpeptidase also showed a marked increase but ]iver soluble of those did not change. Alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase of serum were strikingly increased after total ligation of bile duct, but they were increased moderately in partially ligated rats. And the activities of the three enzymes were markedly decreased in the liver of totally and partially bile ducts ligated rats. Incorporation rates of amino acid mixture-(U)-14C into both serum protein and liver protein showed a significant increase after the total bile duct ligation in rats and reverse effect was observed after the administration of actinomycin D. In the case of partial ligation, the incorporation rate showed a slight increase in ligated lobe compared with patent lobe of liver. The above results showed that bile duct ligation might cause an early increase in synthesis of hepatic alkaline phosphatase, however, the increase in synthesis of 5'-nucleotidase and gamma-glutamyl transpeptidase is delayed until the phase of bile duct proliferation. The early changes within 1 day in serum activities of 5f-nucleotidase and gamma-glutamyl transpeptidase seemed to be caused by alteration of membrane permeability of hepatic cell.